1. prepare a beaker glass 1000 ml, Spatula, Aquadest, glass, Stirrer Hot plate stirrer, medium PDA and Chloramphenicol Selective Supplement
2. Weigh 39 grams medium PDA into a beaker glass 1000 ml.
3. Add aquadest to 1000 ml using a spatula, stir until dissolved.
4. Add Chloramphenicol Selective Supplement 2 vial. For 1000 ml of solution PDA.
5. Move into the sample bottle 500 ml with Cap meeting, lightly coat the cover with aluminum foil. Autoclave the medium at a temperature of 121 ° C for 20 minutes.
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