Ukur contoh air sebanyak 25 ml atau

Ukur contoh air sebanyak 25 ml atau timbang contoh padat dan semi padat sebanyak 25 g dan masukkan dalam wadah steril
Untuk preparasi sampel yang akan dianalisa
Buat larutan pengencer yang terdiri dari Butterfield’s Phosphate – Buffered Dilution Water, Buffered Pepton Water, dan media Plate Count Agar / PCA.
Untuk menyiapkan larutan pengencer dan media yang digunakan dalam menganalisa sampel
Siapkan pengenceran dari 10-2,10-3,10-4 atau pengenceran lain yang sesuai
Untuk mengencerkan sampel
Dari sampel yang homogen dipipet 10 ml ke dalam 90 mL pengencer ( 1: 9 ), . Kocok semua pengenceran 25 kali dalam 30 cm selama 7 detik.
Untuk menghindari sampel berbusa
Pipet 1 ml setiap pengenceran ke dalam cawan petri yang terpisah dan lakukan secara duplo, kocok kembali larutan di dalam botol pengencer jika lebih dari 3 menit sebelum dipipet ke dalam cawan petri
Untuk analisa sampel
Tambahkan 12-15 ml media PCA (media sudah didinginkan pada suhu 45±1) untuk setiap pengenceran
Untuk pemupukan pada sampel
Campurkan media dan sample dengan cara menggerakan petri bolak balik dengan hati-hati secara melingkar pada permukaan yang rata
Untuk meratakan sampel dengan media
Biarkan media memadat, balik cawan petri. Inkubasi selama 48 ±2 jam pada 35. Setelah 48 jam hitung bakteri
Untuk inkubasi,dan mengetahui hasil

Hasil (Inggris) 1:

measuring water samples 25 ml or weigh example of solid and semi-solid at 25 g and put in sterile containers
for the preparation of samples to be analyzed
for diluting solution consisting of Butterfield's phosphate - buffered dilution water, buffered peptone water, and medium plate count order / pca.
to prepare a solution of diluent and media used in analyzing samples
prepared dilution of 10-2,10-3,10-4 or other appropriate dilution to dilute samples

of a homogeneous sample of 10 ml pipetted into 90 ml of diluent (1: 9). Shake all dilutions 25 times within 30 cm for 7 seconds.

to avoid foaming sample pipette 1 ml of each dilution into separate petri dishes and done in duplicate,whipped back in the bottle diluent solution if more than 3 minutes before pipetted into a petri dish for analysis of samples

pca add 12-15 ml of medium (medium had cooled to a temperature of 45 ± 1) for each dilution of the sample for fertilization

mix media and the sample by means of moving back and forth petri carefully in a circle on a flat surface
to flatten the sample with the media
let the media solidified, behind the petri dish. incubation for 48 ± 2 hours at 35. after 48 hours of incubation
bacterial count, and know the results

Hasil (Inggris) 2:

Measure the water sample as many as 25 ml or weigh a solid and semi solid examples of as many as 25 g and enter in a container of sterile
For sample preparation that will be analyzed
Create a solution of diluent composed of Butterfield's Phosphate-Buffered Water, Buffered anti-dilution provisions Pepton Water, and the media in order to Count Plate/PCA.
for preparing aqueous diluent and media used in analyzing the samples
Prepare a dilution of 10-2, 10-3, 10-4 or other appropriate dilution
To dilute the samples
homogeneous samples Of dipipet 10 ml into 90 mL of the diluent (1: 9). Shake all the dilution 25 times in a 30 cm for 7 sec.
to avoid foaming
sample Pipette 1 ml of each dilution in separate petri dish and do on a duplo, beat back the solution in the bottle-thinning if more than 3 minutes before dipipet into the petri dish samples for analysis of

Add 12-15 ml PCA media (media has been cooled at a temperature of 38 ± 1) for each dilution
For fertilization in samples
Combine media and sample by way of moving the petri back and forth gently in a circular on a flat surface
To flatten the samples with media
Let media solidified, behind the petri dish. Incubation for 48 hours at 37 ± 2. After 48 hours incubation
for bacteria count, and know the results