1. Gram Staining• Clean the glass body with alcohol, the fixation on the Bunsen flame until it is dry and free of grease• Create a brand of thin glass bacteria on objects, dry it and then the fixation with missed it over the flame of the Bunsen• Add the brand with Crystal violet (G A) allow for 30 minutes and wash it with running water until clear and dry• Add iodine solution (Gram B, solution of Mordan) leave for 30 seconds, rinse with running water and pat dry• Do dekolorisasi by adding ethyl alcohol with the brand of 95% for 10-20 seconds. To stop the activity dekolorisasi aliri with water until clear and dry • Add Safranin (an) on brand of bacteria for 20-30 seconds
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