prepare sample bottles, plastic clips sterile, gas burner, cap sterile, dry ice. for preparation / preliminary analysis using gloves
bottle then spray your hands and work equipment (laboratory suit) with 70% alcohol to ensure that no contamination will occur at the time of sampling bottles
prepare medium sma / pca and conditioning your pda liquid medium at a temperature of 45 ° C for the preparation of the medium that will be used as a medium for analysis
preheat the edge / neck of the bottle by using the gas burner.panaskan also the edge / medium bottle neck on the bottle sma / pca, pda with a gas burner. for sterilization in a sample bottle and pour a bottle of medium
sma approximately 5 ml of medium in a sample bottle.averaged on all parts of the bottle until there is a part that jgn terlewat.tutup bottle using a sterile cap. to the process of pouring the media and media equalization
bury the bottle to within the container / box containing cold water, roll the bottle quickly and evenly until the entire media sma on the inside and the whole bottle.incubation in an incubator with a temperature of 35 ° C (48 hours / 2 days) for sample analysis (package) and incubating the sample bottle
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