Buat larutan pengencer dan media ya

Buat larutan pengencer dan media yang terdiri dari Buffered Peptone Water, Lauryl Tryptose Broth (LSTB), EC Broth, Levine's cosin-methylene blue (L-EMB) agar, Metil red Voges-Proskauer Broth (MR-VP Broth), Tryptose broth, Indikator Metil Red, KOH 40%
Untuk persiapan analisa sampel
Siapkan pipet, tips, cawan petri yang telah dibungkus ke dalam autoklaf
Untuk mensterilkan alat yang akan digunakan untuk analisa, untuk menjamin alat tersebut tidak mengkontaminasi sampel
Timbang sampel padat dan semi padat sebanyak 50 gram atau 50 ml sampel cair secara aseptik, kemudian masukkan dalam wadah steril.
Untuk menyiapkan sampel yang akan dianalisa
Siapkan pengenceran desimal dengan pengencer butterfield’s phosphate steril atau BPW steril, angka dari pengencer yang disiapkan tergantung dari kepadatan coliform
Untuk menduga keberadaan E.Coli dalam sampel
Kocok semua suspensi 25 kali atau kocok mengunakan vortex selama 7 detik. Jangan gunakan pipet untuk memindahkan

Hasil (Inggris) 1:

Create a solution of diluent and media Buffered Peptone Water consists of, Lauryl Tryptose Broth (LSTB), EC Broth, Levine's my Cosin.He loves to-methylene blue (L-EMB) agar, methyl red-Voges Proskauer Broth (MR-VP Broth), Tryptose broth Methyl Red indicator, KOH 40%For the preparation of the analysis samplePrepare the dropper, tips, petri dish that has been wrapped into an autoclaveTo sterilize the tools that will be used for analysis, to ensure that the tool does not contaminate the samplesWeigh the solid and semi-solid samples as much as 50 grams or 50 ml of liquid sample by aseptik, then enter in the sterile container.To prepare the samples will be analyzedPrepare decimal dilution with sterile diluent butterfield's phosphate or sterile, numbers of BPW diluent are prepared depending on the density of coliformFor a presumes the presence of e. Coli in the samplesShake all suspension whipped 25 times or using vortex for 7 seconds. Do not use the Eyedropper to move

Hasil (Inggris) 2:

Create a diluent solution and media consisting of Buffered Peptone Water, Lauryl Tryptose Broth (LSTB), EC Broth, Levine's cosin-methylene blue (L-EMB) agar, Methyl red Voges-Proskauer Broth (MR-VP Broth), Tryptose broth, Indicators of Methyl Red, 40% KOH
preparation for sample analysis
Prepare pipettes, tips, petri dish which has been wrapped into the autoclave
to sterilize the tools that will be used for analysis, to ensure the device does not contaminate the sample
Weigh solid and semi-solid samples of 50 grams or 50 ml liquid sample aseptically, then enter it in a sterile container.
To prepare the sample to be analyzed
Prepare decimal dilutions with Butterfield's phosphate diluent sterile or sterile BPW, number of diluent prepared depending on the density of coliform
To infer the presence of E. coli in samples
Beat all suspension 25 times or shake using a vortex for 7 seconds. Do not use a pipette to transfer