In addition, utilization of soursop leaves can with extraction. Extraction of the chemical content of withdrawal is an activity that can dissolve so that apart from the material is insoluble with solvent liquid. The active compounds contained in various simplisia can be classified into the volatile oil, principal, flavonoida, and others. With the known active compounds simplisia contained solvent selection will make it easier and how proper extraction (Dit POM, 2000).The extraction can be done by maceration, perkolasi, reflux, sokletasi, and infundasi methods, dekok.Test SitoksisitasCurrently, studies on the active compounds from natural materials is highly encouraged. But many of the ingredients of natural medicine who have been isolated, characterized and published without test activity continued with Biologics. The activity of plant biology is unknown to many years. This is because due to the search for compounds that have pharmacological activity often use assay activity. Therefore it takes a test the activity in General is simple, easy and cheap yet reliable and can detect the presence of compounds that have biological activity is widely found in the extract, fractions and isolates (Rahman, 1991). Some preliminary tests who qualify – the terms above are: method of Potato disks, Brine Shrimp Lethality Test (BST) and test of Lemna minor l. (Meyer, 1982; McLaughlin, 1998).2.3.1 methods of Potato disks (crown gall tumor inhibiting)Method of Potato disks (crown gall tumor inhibiting) crown gall Tumors of plants is a disease caused by a specific strain of Agrobacterium tumefaciens gram-negative bacteria. There are similarities between the mechanism of onset of tumors in plants and in animals, compounds that can inhibit tumor growth in plants can also serve as antitumor in animals. This test is a preliminary test is simple to find anticancer compounds from natural ingredients. Inhibition of the growth of crown gall Tumors on potato discs by extract of natural ingredients, natural material extracts showed that the active (McLaughlin, 1998).
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