Pasang peralatan penyaring membran

Pasang peralatan penyaring membran yang telah disterilkan terlebih dahulu dan hubungan dengan vakum
Untuk menghindari kontaminasi sehingga digunakan alat yang telah disterilkan terlebih dahulu
Masukkan 100 ml cuplikan (sampel) ke dalam corong dari alat penyaring dengan menggunakan pipet atau gelas ukur steril. Saring sampel seluruhnya dengan menggunakan vakum.
Untuk menyaring (cuplikan) sampel melalui membran
Bilas seluruh permukaan dalam corong penyaring dengan 100 ml air pengencer atau air suling steril dan saring cairan pembilas. Hentikan vakum setelah pembilasan selesai.
Untuk memastikan semua cuplikan tersaring (membersihkan sisa sampel yang terdapat pada corong)
Buka peralatan penyaring dan angkat membran penyaring dengan pinset yang steril
Untuk menghindari kontaminasi pada sampel
Letakkan membran penyaring di atas media violet red bile agar dalam cawan petri (Usahakan jangan ada gelembung di bawah membran).
Untuk tempat pertumbuhan (pembiakan) bakteri
Inkubasi cawan dengan posisi terbalik pada 36 ± 1⁰C selama 24-48 jam
Untuk menumbuhkan bakteri

Hasil (Inggris) 1:

pairs of membrane filter equipment that has been sterilized beforehand and relationships with vacuum
to avoid contamination so used tool that has been sterilized beforehand
fill 100 ml samples (samples) into the funnel of the filter device using a sterile pipette or measuring cup. strain the total sample by using a vacuum.
to filter (footage) of the sample through the membrane
rinse the entire surface of the filter funnel with 100 ml dilution water or sterile distilled water and strain the liquid rinse. stop vacuum after flushing is complete.
to ensure all samples are filtered (clean the rest of the samples contained in the funnel)
open and lifting equipment filter membrane filter with sterile tweezers
to avoid contamination of the sample
put on a membrane filter medium violet red bile that in a petri dish (do not try no bubbles under the membrane).
To the place of growth (breeding) incubation of bacteria
cup upside down at 36 ± 1 ⁰ C for 24-48 hours to grow the bacteria

Hasil (Inggris) 2:

Install the membrane filter equipment has been sterilized beforehand and relationship with vacuum
to avoid contamination and so used the tools that have been sterilized in advance
Enter 100 ml samples (samples) into the funnel of the filter by using the eyedropper tool or a sterile measuring cup. The sample filter entirely with the use of a vacuum.
(Excerpts) to filter the sample through a membrane Rinse entire surface in
funnel filter with 100 ml of water or diluent of sterile distilled water and strain the liquid pembilas. Stop the vacuum after the flushing is complete.
to make sure all samples filtered (clean the rest of the samples found on the funnel)
filter equipment and lift Open the membrane filter with sterile tweezers
To avoid contamination in the samples
Put the membrane on top of filter medium violet red bile agar in a petri dish (So no bubbles under the membrane).
For bacterial growth (breeding)
Incubation bowls with inverted position at 35 ± 1 ⁰ C for 24-48 hours for the bacteria to grow