1. Siapkan beaker glass 1000 ml, Sp

1. Siapkan beaker glass 1000 ml, Spatula, Aquadest, Pengaduk kaca, Hot plate stirrer, medium PCA
2. Timbang 22,5 gr medium PCA ke dalam beaker glass 1000 ml
3. Tambahkan aquadest sampai 1000 ml , Aduk dengan menggunakan spatula sampai larut.
4. Panaskan pada hot plate stirrer sampai larut sempurna /homogen (warna jernih ). Tuang pada botol steril 500 ml, tutup rapat lapisi bagian cap botol dengan alumunium foil.
5. Autoclave medium pada suhu 121°C selama 20 menit.

Hasil (Inggris) 1:

1. prepare 1000 ml glass beaker, spatula, distilled water, glass stirrer, hot plate stirrer, medium pca
2. Weigh 22.5 grams of medium pca into 1000 ml glass beaker
3. add distilled water to 1000 ml, using a spatula stir until dissolved.
4. heat on a hot plate stirrer until completely dissolved / homogeneous (clear color). Pour in 500 ml sterile bottles,cover tightly seal the bottle with a piece of aluminum foil stamp.
5. medium autoclave at 121 ° C for 20 minutes.

Hasil (Inggris) 2:

1. prepare a beaker glass 1000 ml, Spatula, Aquadest, glass, Stirrer Hot plate stirrer, medium PCA
2. Weigh 22.5 grams medium PCA into a beaker glass 1000 ml
3. Add aquadest to 1000 ml using a spatula, stir until dissolved.
4. Heat on a hot plate stirrer until it dissolves perfectly/homogen (clear color). Pour in a bottle of 500 ml, sterile lightly coat the cover tightly with aluminum foil bottle cap.
5. Autoclave the medium at a temperature of 121 ° C for 20 minutes.