Penelitian ini merupakan penelitian

Penelitian ini merupakan penelitian deskriptif eksploratif dengan observasi laboratoris yang dilakukan dalam bulan November-Desember 2015. Analisis protein menggunakan crude protein hasil isolasi protein transmembran spermatozoa sapi Aberdeen angus yang kemudian dilakukan analisis dengan menggunakan metode elektroforesis SDS-PAGE yang telah memunculkan pita protein spesifik dengan berat molekul 64 kDa sebagai calcium binding protein dan 33 kDa sebagai protein tirosin fosforilasi. Pita tersebut kemudian dipotong dan dilakukan anilisis dengan metode elektroelusi. Protein hasil elektroelusi dilakukan perhitungan untuk mencari ATP yang bereaksi dan unit aktivitas fosforilasi. Didapatkan hasil, protein pada berat molekul 64 kDa disfosforilasi ATP sebesar 41 ppm dengan unit aktivitas 119,013062x103 µmol/ml/menit dan protein dengan berat molekul 33 kDa telah memfosforilasi ATP sebesar 27 ppm dengan unit aktivitas 78,374456x103 µmol/ml/menit. Besarnya jumlah ATP yang difosforilasi mengindikasi bertambahnya unit aktivitas fosoforilasi serta bertambahnya kecepatan protein dalam memfosforilasi protein.

Hasil (Inggris) 1:

This research is a descriptive exploratory research with the laboratoris observation conducted in November-December 2015. Analysis of proteins using crude protein transmembrane protein isolation results spermatozoa Aberdeen angus cows are then conducted the analysis using the method of electrophoresis SDS-PAGE which has raised specific protein bands with molecular weights 64 kDa as calcium binding protein 33 kDa and as protein tyrosine phosphorylation. The Ribbon is then cut and done anilisis with the elektroelusi method. Protein results elektroelusi calculation is performed to locate the ATP that react and unit activity of phosphorylation. Obtained results, a protein on the 64 kDa molecular weight disfosforilasi ATP of 41 ppm with the unit activity 119, 013062x103 µmol/ml/min and protein 33 kDa molecular weight have phosphorylates ATP of 27 ppm with the activity unit 78, 374456x103 µmol/ml/minute. The large amount of ATP that difosforilasi indicates the increase of the activity of the fosoforilasi unit as well as increasing the speed of protein in phosphorylates proteins.

Hasil (Inggris) 2:

This study is a descriptive exploratory study with laboratory observations were conducted in November-December 2015. Analysis of the protein using crude protein transmembrane protein isolated spermatozoa Aberdeen angus beef which is then analyzed using SDS-PAGE electrophoresis method that has led to specific protein bands with heavy 64 kDa molecule as a calcium binding protein and 33 kDa as protein tyrosine phosphorylation. The ribbon is then cut and performed analysis, with methods of electroelution. Protein electroelution calculation result to find that react ATP and phosphorylation activity unit. The results obtained, the protein molecular weight 64 kDa disfosforilasi ATP at 41 ppm with 119,013062x103 activity unit mol / ml / min and a protein with a molecular weight of 33 kDa was phosphorylate ATP by 27 ppm with 78,374456x103 activity unit mol / ml / min. The large amount of ATP that is phosphorylated indicates increasing unit fosoforilasi activity and increased speed phosphorylate proteins in protein.