Prepare the pipette and tip that has been wrapped into an autoclave to sterilize
tools that will be used for analysis, to ensure that the tool does not contaminate the samples
take 1 ml of the suspension of each dilution and squirt in the middle of a flat surface on the petrifilm
Align using the plastic spreader and leave for 1 minute
For leveling suspension (sample)
Incubation petrifilm with horizontal position for 24 ± 2 h at 35 ± 1 ⁰ C (not stacking the petrifilm more than 20 vials)
Count bacteria to grow the petrifilm plate numbers as the value
total (TPC)
Add incubation for 24 ± 2 hours (total of 48 ± 2 HR)
Count the colonies that formed on the petrifilm plate numbers as the value
total (units of colonies per g/ml)
Sedang diterjemahkan, harap tunggu..
