Preserving microbes are divided into two, namely the preservation of long-term and short-term preservation. Short term storage of microbes is done by moving the short term e.g. periodically once a month from old media to new media. This technique requires a lot of time and energy [6]. Usually, the short term preservation use media so that is so lopsided, in order for the Cup, to skewer or in solid media semia (tube), and preservation was done by means of cooling. Some simple storage techniques that are effective for short-term or isolates, storage medium, and his usual is not suitable for long term storage. Among these techniques is storage in mineral oil, liquid paraffin, sterile water, sterile soil, beads porcelain, slab of gelatin, and P2O5 in a vacuum. Although not widely used, these techniques require only simple equipment and easy to obtain, making it useful for agencies that do not have sophisticated equipment [7].
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