Firstly five different locations were selected in both districts (Fig. 1). Then 30 A. dorsata worker bees were obtained per each site from both districts (Nankana and Narowal). Plastic bottles containing 50% alcohol were used for sample preservation indicating sam- ple and hive number, collecting site with date and kept at 4 C for extraction of DNA (Issa et al., 2013). Identification of honey bee species was carried out by using key given in Asian Honey Bees (Oldroyd and Wongsiri, 2006).
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