Clear zone (halozone) is an early sign to determine the ability of MPF in dissolving phosphate. The wider the clear zone, qualitatively can be considered as a sign of the ability of MPF dissolving phosphate in the media grew. Similarly increasingly clear / bright clear zone indicates phosphate solubilizing intensified. Width / midline colonies and a clear zone can be measured, in general, the greater the value ratio between the center line of clear zone: the center line of the colonies, showing the ability of MPF in dissolving phosphate qualitatively greater, although this is not enough to describe the ability of MPF in dissolving phosphate actual (Nautiyal, 1999).
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