Concerning the analytical method th

Concerning the analytical method that includes the alkaline
hydrolysis step, the results were markedly different. The saponification was observed to affect to precision, since the RSD values were
higher, both intra-day and inter-day. Specifically, the observed
RSD values varied from 14.5% to 26.4% (repeatability) and from
24.6% to 27.6% (reproducibility). Saponification is widely used for
carotenoid analysis as it can simplify the chromatograms by removing unwanted lipids and chlorophylls. More often they are used
in samples were CARS are esterified. As a result of the hydrolysis free xanthophylls are analyzed instead of the several esters of
them that may be present. However, the reaction also extends the
analysis time and is known to lead to losses of some carotenoids,
the extent of which depend on the chemical structure of the
CARS and saponification conditions like length, concentration of
alkali and temperature [40,41]. In fact, it is recommended that the
saponification step is avoided whenever possible. For instance it
is unnecessary in samples like leafy vegetables and tomatoes that
do not contain high quantities of CARs esters and are not fatty
materials [42,43].
The comparison of the results obtained in the present study
with those reported by other authors is not straightforward whatsoever due to differences in the samples and validation approaches.
For instance, lower RSD values have been reported for these compounds in other studies, although such results were obtained by
analyzing a mixture of standards rather than by considering both
the extraction and HPLC procedures [28]. In such case only the precision of the chromatographic method was taken into account and
not that of the extraction process, which is more likely to produce
analyte losses. On the other hand, there are studies in which the
validation parameters were obtained by considering the extraction method without saponification, a reaction which is known to
result in important CARS losses, as already discussed. For instance,
Gleize et al. [23] evaluated the precision of a methodology without
saponification for plasma samples (where CARS are not esterified),
reporting RSD values lower than 13%. In another study, RSD values
under 10% were reported for samples spiked with standards just
prior to the saponification step, such that neither the effect of the
matrix nor that of the saponification were considered [39]

Hasil (Inggris) 1:

Concerning the analytical method that includes the alkalinehydrolysis step, the results were markedly different. The saponification was observed to affect to precision, since the values were athigher, both intra-day and 2-day. Specifically, the observedAt values varied from 14.5% to 26.4% (repeatability) and from24.6% to 27.6% (reproducibility). Saponification is widely used forcarotenoid analysis as it can simplify the chromatograms by removing unwanted lipids and chlorophylls. More often they are usedin samples were CARS are are esterified. As a result of the hydrolysis of free xanthophylls are analyzed instead of the several esters ofthem that may be present. However, the reaction also extends theanalysis time and is known to lead to losses of some carotenoids,the extents of which depend on the chemical structure of theCARS and saponification conditions like length, the concentration ofalkali and temperature [40,41]. In fact, it is recommended that thesaponification step is avoided whenever possible. For instance itis unnecessary in samples like leafy vegetables and tomatoes thatdo not contain high quantities of CARs and are not fatty estersmaterials [42,43].The comparison of the results obtained in the present studywith those reported by other authors is not straightforward due to differences whatsoever in the samples and validation approaches.For instance, lower at values have been reported for these compounds in other studies, although such results were obtained byanalyzing a mixture of standards rather than by considering boththe extraction and HPLC procedures [28]. In such case only the precision of the chromatographic method was taken into account andnot that of the extraction process, which is more likely to produceanalyte losses. On the other hand, there are studies in which thevalidation parameters were obtained by considering the extraction method without saponification, a reaction which is known toresult in important losses, u.s. CARS already discussed. For instance,Gleize et al. [23] evaluated the precision of a methodology withoutsaponification for plasma samples (where CARS are not are esterified),reporting at values lower than 13%. In another study, at valuesunder 10% were reported for samples spiked with standards justprior to the saponification of the step, such that "neither the effect of thematrix nor that of the saponification were considered [39]

Hasil (Inggris) 2:

Concerning the analytical method that includes the alkaline
hydrolysis step, the results were markedly different. The saponification was observed to Affect to precision, since the RSD values ​​were
higher, both intra-day and inter-day. Specifically, the observed
RSD values ​​varied from 14.5% to 26.4% (repeatability) and from
24.6% to 27.6% (reproducibility). Instant confirmation saponification is used for
carotenoid analysis as it can simplify the chromatograms by removing unwanted lipids and chlorophylls. More Often they are used
in samples were CARS are esterified. As a result of the hydrolysis free xanthophylls are Analyzed instead of the Several esters of
them that may be present. However, the reaction Also extends the
analysis time and is known to lead to losses of some carotenoids,
the extents of the which depend on the chemical structure of the
CARS and saponification conditions like length, concentration of
alkali and temperature [40,41]. In fact, it is recommended that the
saponification step is avoided Whenever possible. For instance it
is unnecessary in samples like leafy vegetables and tomatoes that
do not contain high quantities of CARs esters and are not fatty
materials [42,43].
The comparison of the results Obtained in the present study
with Reviews those reported by other authors is not straightforward whatsoever due to differences in the samples and validation approaches.
For instance, the lower RSD values ​​have been reported for these compounds in other studies, Although Reviews such results were Obtained by
analyzing a mixture of standards rather than by considering both
the extraction and HPLC procedures [ 28]. In Reviews such case only the precision of the chromatographic method was taken into account and
not that of the extraction process, the which is more Likely to produce
analyte losses. On the other hand, there are studies in the which the
validation parameters were Obtained by considering the extraction method without saponification, a reaction the which is known to
result in important CARS losses, as already Discussed. For instance,
Gleize et al. [23] evaluated the precision of a methodology without
saponification for plasma samples (where the CARS are not esterified),
reporting RSD values ​​lower than 13%. In another study, the RSD values
​​under 10% were reported for samples spiked with standards just
prior to the saponification step, Reviews such that Neither the effect of the
matrix nor that of the saponification were Considered [39]